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Bioprocess Control Station / Bioprocessing

BIOne 1250

Bioprocess Control Station

Distek, Inc. has revolutionized benchtop scale bioreactors with the release of our BIOne 1250 bioprocess control station for both mammalian and microbial models. The sleek appearance and intuitive touchscreen interface are only the beginning. The BIOne 1250 is designed to be the easiest to use and most capable stand-alone controller on the market. Control all bioreactor parameters directly from the BIOne 1250 interface, or, for remote control, use our optional OPC server and communicate with your choice of SCADA system. Seamlessly transition between our autoclavable and the BIOne single-use bioreactor in seconds. CLICK HERE to view the product specifications.

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Configurable For Your Lab

Designed to fit your needs, the BIOne controller can be customized to conform to your lab design:

Rotameters or Auto Flow Control
- Up to 5 Gas Control Modules
Left or Right Side Setup
Optical pH or Standard
Single-Use or Autoclavable Bioreactors
Mammalian or Microbial Models and much more…

Data Trending

Visually Inspect Your Run
Add ‘Event’ Notes
- Note Anomalies
- Call Attention to Specific Time Points
- Events Populate in Exported Data
Trend up to 8 Parameters
Compare 2 Runs Simultaneously

Single-Use or Autoclavable Bioreactors

The BIOne 1250 can seamlessly transition between autoclavable and our BIOne single-use bioreactor:

Magnetic Coupling Drive
- Fully Contained
- Low Maintenance
- Eliminates Contamination Point
Single-Use or Stainless Steel Headplate
Single-Use (2L & 5L) or Autoclavable (2L, 5L & 10L)
Stable & Secure

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BIOne 1250 Left Side with Autoclavable Bioreactor

BIOne 1250 Trend Screen

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BIOne Autoclavable Bioreactor for Cell Culture

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BIOne 1250 Right Side with BIOne Single-Use Bioreactor

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BIOne 1250 Overview Screen

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BIOne 1250 Left Side with BIOne Single-Use Bioreactor

BIOne 1250 Overview Screen with pH Popup

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BIOne Autoclavable Bioreactor for Microbial

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BIOne 1250 Right Side with Autoclavable Bioreactor

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Applications

Characterizing the Effects of Antifoam C Emulsion on kLa within the BIOne SUB

To better understand and characterize the effects of antifoam supplementation on oxygen mass transfer, our team performed a dose-response evaluation of Antifoam C in both drilled-hole sparger and microsparger BIOne Single Use Bioreactors (SUBs). Within the drilled-hole sparger system, results were consistent with previously reported data. A 40 – 50% overall reduction in k_La was observed upon treatment with 30 ppm Antifoam C emulsion. In contrast, no effects from the antifoam treatment were observed within the microsparger bioreactor system. Overall, these results suggest that use of a microsparger may support consistent oxygen mass transfer within high-foaming upstream bioprocesses where antifoam supplementation is required. CLICK HERE TO VIEW THE WHITE PAPER.

Increasing Oxygen Transfer within the BIOne Single-Use Bioreactor using a Microsparger

Determining the appropriate bioreactor system configuration for an individual upstream bioprocess can be a challenging task. The nuanced details which differentiate the variety of mammalian cell culture processes will ultimately mean that certain bioreactor configurators will be preferable for specific upstream applications .In this work, we used two types of BIOne Single-Use Bioreactors (SUBs) to characterize the potential difference in oxygen mass transfer between two commonly used sparger types: drilled-hole spargers and microspargers. CLICK HERE TO VIEW THE WHITE PAPER.

Upstream Process Development with a kLa Criterion Within the BIOne Single-Use Bioreactor

The QbD directive from the FDA states that extensive bioprocess characterization and understanding should be essential elements of future bioprocess developmental work. As such, we performed novel HEK-293 batch process development within the BIOne SUB using a k_La central criterion to define operations definitions for our volume dependent parameters.

The success of this study supports the suitability of k_La as central criterion for bioprocess development. Additionally, these results demonstrated that the BIOne SUB can be highly suitable for novel upstream process developmental work. CLICK HERE TO VIEW THE WHITE PAPER.

Recognizing the Potential Impact of Agitation Directionality On Oxygen Mass Transfer Through a kLa Evaluation Within the BIOne Single-Use Bioreactor

Detailed understanding of the effects of agitation on oxygen mass transfer within a bioreactor system can help facilitate successful upstream bioprocess development and characterization. As shown in this work, the directionality of the axial flow may influence the overall k_La of a system. Process scientists, engineers, and researchers may find value in considering directionality of the axial flow as a process parameter during their own upstream bioprocess characterization and development work. CLICK HERE TO VIEW THE WHITE PAPER.

Advancing the Robust Manufacture of T-Cell Therapies through the Application of Stirred Tank Bioreactors

Stirred-tank bioreactors present an option for the expansion of primary human T-Cells that has previously not been pursued due to concerns about shear stress. Small scale studies have shown that impeller driven mixing stirred-tank bioreactors do not increase cell death or limit expansion potential. Translating the learnings from those small scale studies to the autologous manufacturing scale has shown that the BIOne stirred-tank bioreactor produces a consistent culture environment and high cell yields. The BIOne significantly outperformed rocking bioreactor systems in both total yield and production efficiency. CLICK HERE TO VIEW THE POSTER.

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Distek provides quotations on price and availability upon request. Quotations are processed by our Inside Sales Team 5 days a week from 8:30 to 5:00 EST. Typical turnaround for all quotes is 24-48 hours. Should you have any questions please direct them to orders@distekinc.com.

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