Stay on Target: Improving Process Control through Strategic Cascade Feedback Loop Tuning
In this work, we present an overview of the operation of cascade controllers. We discuss the utility of such systems within upstream bioprocess manufacturing. We then demonstrate this utility with a practical application example. We execute a model process under different sets of cascade configurations and record both the magnitude of pH fluctuations and volumes of pH adjustment media required to maintain setpoint throughout the process duration. Results of this study demonstrate the potential for improved process control though iterative cascade loop tuning. Scientists and engineers might consider adopting the techniques outlined in this study to evaluate and improve cascade feedback control within their own bioprocess applications. CLICK HERE TO VIEW THE WHITE PAPER.
The Significance of PID Tuning within Biopharmaceutical Processes
The intent of this information is to provide foundational knowledge of the subject matter to support PID tuning efforts within biopharmaceutical laboratories. We conclude this work with a practical example that demonstrates the benefits of PID tuning. In this application example, heating regression PID parameters for a highly complex upstream process model are optimized using a closed-loop feedback tuning method. The resulting process demonstrates the potential for improved process control through the execution of strategic PID parameter tuning. CLICK HERE TO VIEW THE WHITE PAPER.
Strategically Defining Operational Parameters for Microcarrier Upstream Process within a SUB
In this work, we present an elegant and scalable method for the onboarding of a simple, three-dimensional, microcarrier-based hMSC expansion bioreactor process. Results of the study showed an 11.9-fold culture expansion at 96.5% viability after a 6-day culture period. These data demonstrate the suitability of the process for supporting the intricate needs of hMSC cultures. This work can serve as a model for teams looking to successfully onboard three-dimensional hMSC expansion processes onto their own laboratory benches. CLICK HERE TO VIEW THE WHITE PAPER.
Characterizing Mixing Dynamics within a Single-Use Stirred Tank Reactor (STR) System
Bioprocess Development in Stirred Tank Reactor (STR) systems often requires the execution of thorough mixing validation studies. Increase your team’s efficiency by performing these studies at small volumes within benchtop models, such as the Distek BIOne Single-Use Bioreactor. CLICK HERE TO VIEW THE WHITE PAPER.
Demonstration of the Effectiveness of Design of Experiment Methodology for Strategic Upstream Process Development
Ready to expand the characterization of your Upstream Bioprocess through Design of Experiments (DoE), but unsure of how to start? Check out Distek’s latest white paper for a DoE overview that can help guide your team through the process.
In this work, we demonstrated the suitability of response surface DoE designs for both the optimization of a transient gene expression process and the characterization of oxygen mass transfer within a 2-L BIOne Single-Use Bioreactor System. CLICK HERE TO VIEW THE WHITE PAPER.
Characterizing the Effects of Antifoam C Emulsion on kLa within the BIOne SUB
To better understand and characterize the effects of antifoam supplementation on oxygen mass transfer, our team performed a dose-response evaluation of Antifoam C in both drilled-hole sparger and microsparger BIOne Single Use Bioreactors (SUBs). Within the drilled-hole sparger system, results were consistent with previously reported data. A 40 – 50% overall reduction in kLa was observed upon treatment with 30 ppm Antifoam C emulsion. In contrast, no effects from the antifoam treatment were observed within the microsparger bioreactor system. Overall, these results suggest that use of a microsparger may support consistent oxygen mass transfer within high-foaming upstream bioprocesses where antifoam supplementation is required. CLICK HERE TO VIEW THE WHITE PAPER.
Increasing Oxygen Transfer within the BIOne Single-Use Bioreactor using a Microsparger
Determining the appropriate bioreactor system configuration for an individual upstream bioprocess can be a challenging task. The nuanced details which differentiate the variety of mammalian cell culture processes will ultimately mean that certain bioreactor configurators will be preferable for specific upstream applications .In this work, we used two types of BIOne Single-Use Bioreactors (SUBs) to characterize the potential difference in oxygen mass transfer between two commonly used sparger types: drilled-hole spargers and microspargers. CLICK HERE TO VIEW THE WHITE PAPER.
Upstream Process Development with a kLa Criterion Within the BIOne Single-Use Bioreactor
The QbD directive from the FDA states that extensive bioprocess characterization and understanding should be essential elements of future bioprocess developmental work. As such, we performed novel HEK-293 batch process development within the BIOne SUB using a kLa central criterion to define operations definitions for our volume dependent parameters.
The success of this study supports the suitability of kLa as central criterion for bioprocess development. Additionally, these results demonstrated that the BIOne SUB can be highly suitable for novel upstream process developmental work. CLICK HERE TO VIEW THE WHITE PAPER.
Recognizing the Potential Impact of Agitation Directionality On Oxygen Mass Transfer Through a kLa Evaluation Within the BIOne Single-Use Bioreactor
Detailed understanding of the effects of agitation on oxygen mass transfer within a bioreactor system can help facilitate successful upstream bioprocess development and characterization. As shown in this work, the directionality of the axial flow may influence the overall kLa of a system. Process scientists, engineers, and researchers may find value in considering directionality of the axial flow as a process parameter during their own upstream bioprocess characterization and development work. CLICK HERE TO VIEW THE WHITE PAPER.
