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Autoclavable Vessel / Bioprocessing

Fermentation

Autoclavable Fermentation System

Engineered to support superior process robustness, within the Distek Autoclavable Fermentation systems, form follows function.

Intelligently designed system components ensure sufficient mass and thermal transfer to support the needs of even the most demanding microbial bioprocesses.

Available in vessel sizes from 2L through 10L volumes, the BIOne Autoclavable Fermentation product line has the perfect option to support your laboratory team’s own unique upstream bioprocess needs.

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Working Volume from 750mL to 10L

2L Autoclavable

Total Volume – 3L
Working Volume – 750mL to 2L

5L Autoclavable

Total Volume – 7L
Working Volume – 1.5L to 5L

10L Autoclavable

Total Volume – 13L
Working Volume 2.3L to 10L

17+ Headplate Port Openings

17+ headplate port openings for complete process versatility.

2L Autoclavable
- 5 x M18 / 2 x M12 / 7 x M10 / 3 x M6)
5L Autoclavable
- 6 x M18 / 2 x M12 / 12 x M10
10L Autoclavable
- 6 x M18 / 5 x M12 / 10 x M10

Rushton Impeller Design

Rushton impeller design and integrated baffles for superior mass and thermal transfer.

2L Autoclavable
- Rushton Diameter – 58 mm
5L Autoclavable
- Rushton Diameter – 76 mm
10L Autoclavable
- Rushton Diameter – 81 mm

Magnetic Drive Controlled Agitation

Magnetic drive controlled agitation decreases friction and reduces contamination risks.

Integrated Cooling Elements

Integrated cooling elements support precise temperature control.

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BIOne Autoclavable Fermentation System

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Applications

Characterizing the Effects of Antifoam C Emulsion on kLa within the BIOne SUB

To better understand and characterize the effects of antifoam supplementation on oxygen mass transfer, our team performed a dose-response evaluation of Antifoam C in both drilled-hole sparger and microsparger BIOne Single Use Bioreactors (SUBs). Within the drilled-hole sparger system, results were consistent with previously reported data. A 40 – 50% overall reduction in k_La was observed upon treatment with 30 ppm Antifoam C emulsion. In contrast, no effects from the antifoam treatment were observed within the microsparger bioreactor system. Overall, these results suggest that use of a microsparger may support consistent oxygen mass transfer within high-foaming upstream bioprocesses where antifoam supplementation is required. CLICK HERE TO VIEW THE WHITE PAPER.

Increasing Oxygen Transfer within the BIOne Single-Use Bioreactor using a Microsparger

Determining the appropriate bioreactor system configuration for an individual upstream bioprocess can be a challenging task. The nuanced details which differentiate the variety of mammalian cell culture processes will ultimately mean that certain bioreactor configurators will be preferable for specific upstream applications .In this work, we used two types of BIOne Single-Use Bioreactors (SUBs) to characterize the potential difference in oxygen mass transfer between two commonly used sparger types: drilled-hole spargers and microspargers. CLICK HERE TO VIEW THE WHITE PAPER.

Upstream Process Development with a kLa Criterion Within the BIOne Single-Use Bioreactor

The QbD directive from the FDA states that extensive bioprocess characterization and understanding should be essential elements of future bioprocess developmental work. As such, we performed novel HEK-293 batch process development within the BIOne SUB using a k_La central criterion to define operations definitions for our volume dependent parameters.

The success of this study supports the suitability of k_La as central criterion for bioprocess development. Additionally, these results demonstrated that the BIOne SUB can be highly suitable for novel upstream process developmental work. CLICK HERE TO VIEW THE WHITE PAPER.

Recognizing the Potential Impact of Agitation Directionality On Oxygen Mass Transfer Through a kLa Evaluation Within the BIOne Single-Use Bioreactor

Detailed understanding of the effects of agitation on oxygen mass transfer within a bioreactor system can help facilitate successful upstream bioprocess development and characterization. As shown in this work, the directionality of the axial flow may influence the overall k_La of a system. Process scientists, engineers, and researchers may find value in considering directionality of the axial flow as a process parameter during their own upstream bioprocess characterization and development work. CLICK HERE TO VIEW THE WHITE PAPER.

Advancing the Robust Manufacture of T-Cell Therapies through the Application of Stirred Tank Bioreactors

Stirred-tank bioreactors present an option for the expansion of primary human T-Cells that has previously not been pursued due to concerns about shear stress. Small scale studies have shown that impeller driven mixing stirred-tank bioreactors do not increase cell death or limit expansion potential. Translating the learnings from those small scale studies to the autologous manufacturing scale has shown that the BIOne stirred-tank bioreactor produces a consistent culture environment and high cell yields. The BIOne significantly outperformed rocking bioreactor systems in both total yield and production efficiency. CLICK HERE TO VIEW THE POSTER.

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