BIOne Single-Use Fermentor

Agnostic Design / Ready-to-Use Out of the Box

The BIOne Single-Use Fermentor (SUF) is engineered with a disposable headplate welded to a triple-layered liner. 

• Assembled in Distek’s ISO 7 cleanroom with USP Class VI materials and gamma sterilized, minimizes contamination risk. The BIOne SUF is ready-to-use out of the box.
• Closed system removes the need for cleaning, assembly, or autoclave sterilization.
• Available in a 2L volume (10L coming later this year), the BIOne Single-Use Fermentor offers reduced turnaround time and seamless transitions between processes.
• Features an agnostic design compatible with most common bioprocessing controllers.

Optimizing Oxygen Transfer Rate (OTR)

• Maximum Agitation Rate of 1250 RPM
• Dual Rushton Impellers
• Baffle Cage
• Capable of 425 MMOL O2/L/HR at 1 VVM Air Sparge and 1250 RPM Agitation

E. coli Growth Profile: BIOne SUF vs. Control

• E. coli growth profiles were evaluated in a 2L working volume BIOne SUF and glass vessel control for a 22-hour batch process.
• Both systems were operated with identical conditions.
• Similar growth and titer were observed in the BIOne SUF and glass vessel control.
• Performance results indicate that the BIOne Single-Use Fermentor is a suitable bench scale model for microbial fermentation process development and material generation.

Maximum Gas Exchange for Aerobic Microbial Cultures

High capacity sparge and exhaust filters along with a flute sparger with 7 x 1.5 mm holes delivers excellent gas exchange for highly aerobic microbial cultures.

• High Capacity Sparge and Exhaust Filters
• Flute Sparger (7 x 1.5mm holes)

Customizable Above the Headplate

The single-use headplate incorporates many components from an autoclavable system, with several customizable options.

• Non-Invasive DO (Polarographic)
• Two PG-13.5 Universal Sensor Ports.  Popular uses include:
  • Electrochemical pH
  • Biomass, glucose, etc.
• Custom Tubing Configurations and Filters Available
• Optional Integrated Single-Use pH Probe

BIOne Liner Material

The low antioxidant bioprocess film used is comprised of 3 layers, each serving a specific function:

• Outer Layer (LDPE) – Mechanical Strength
• Middle Barrier Layer (EVOH) – Gas Barrier
• Contact Layer (ULDPE) – Cell Culture Compatible, Biologically Inert