Member Center

Additional resources are available in our Member Center. Click on the 'Member Center' button or Member Center on our navigation bar to be redirected.

Member center

Bioprocessing / Single-Use Bioreactor System

BIOne

Single-Use Bioreactor System

Convert your existing benchtop glass bioreactor to a single-use bioreactor in seconds! Distek, Inc. has developed a benchtop scale single-use bioreactor (SUB) for mammalian cell growth, cell therapy, insect cells, and other unique applications performed in a stirred tank bioreactor. The gamma irradiated BIOne SUB is engineered with a disposable headplate welded to a triple-layered liner that can be easily inserted into a non-sterile bioreactor glass vessel, converting it to a disposable SUB within a matter of seconds. Upon media addition, the liner expands and molds to the glass vessel, preserving the geometry of the existing bioreactor. The BIOne SUB’s liner material emulates the large-scale SUBs used in pilot and commercial biopharm production facilities around the world. Performing R & D studies using the most similar material on the market streamlines your tech transfer and can de-risk your process BEFORE transferring to pilot scale.

Contact Sales

Convert Your Glass Vessel to Single-Use in Seconds

The pre-sterilized BIOne system is engineered with a disposable headplate welded to a triple-layered liner that can be easily inserted into a non-sterile bioreactor glass vessel, converting it to a sterile, disposable SUB within a matter of seconds.

2L & 5L Working Volume
Weldable Tubing
USP Class VI Materials
Compatible with Most Manufacturers’ Glass Vessels and Controllers

Multiple Sensor Ports

The single-use headplate contains all of the components of your existing glass system as well as four probe ports including:

Non-invasive DO sensor port
Non-invasive pH sensor port
Two universal sensor ports which can be utilized for 12mm, CO₂, DO, pH, biomass, glucose, etc.

BIOne Liner Material

The low antioxidant bioprocess film used is comprised of 3 layers, each serving a specific function:

Outer Layer (LDPE) – Mechanical Strength
Middle Barrier Layer (EVOH) – Gas Barrier
Contact Layer (ULDPE) – Cell Culture Compatible, Biologically Inert

Convert Your Glass Vessel to Single-Use in Seconds

BIOne SUB

BIOne SUB Headplate

BIOne SUB In Vessel

2020/10/BIOne Headplate with Tubing 900 x 900.jpg

BIOne SUB Headplate with Tubing

2020/10/BIOne Vessel with Media 900 x 900.jpg

BIOne SUB In Vessel with Media

2020/10/BIOne In and Out Vessel 900 x 900.jpg

BIOne SUB In and Out of Vessel

BIOne SUB with Dual Impeller

2020/10/BIOne Headplate Top 900 x 900.jpg

BIOne SUB Headplate (Top View)

BIOne SUB with ATF Perfusion

0 / 0

Applications

Increasing Oxygen Transfer within the BIOne Single-Use Bioreactor using a Microsparger

Determining the appropriate bioreactor system configuration for an individual upstream bioprocess can be a challenging task. The nuanced details which differentiate the variety of mammalian cell culture processes will ultimately mean that certain bioreactor configurators will be preferable for specific upstream applications .In this work, we used two types of BIOne Single-Use Bioreactors (SUBs) to characterize the potential difference in oxygen mass transfer between two commonly used sparger types: drilled-hole spargers and microspargers. CLICK HERE TO VIEW THE WHITE PAPER.

Upstream Process Development with a kLa Criterion Within the BIOne Single-Use Bioreactor

The QbD directive from the FDA states that extensive bioprocess characterization and understanding should be essential elements of future bioprocess developmental work. As such, we performed novel HEK-293 batch process development within the BIOne SUB using a k_La central criterion to define operations definitions for our volume dependent parameters.

The success of this study supports the suitability of k_La as central criterion for bioprocess development. Additionally, these results demonstrated that the BIOne SUB can be highly suitable for novel upstream process developmental work. CLICK HERE TO VIEW THE WHITE PAPER.

Recognizing the Potential Impact of Agitation Directionality On Oxygen Mass Transfer Through a kLa Evaluation Within the BIOne Single-Use Bioreactor

Detailed understanding of the effects of agitation on oxygen mass transfer within a bioreactor system can help facilitate successful upstream bioprocess development and characterization. As shown in this work, the directionality of the axial flow may influence the overall k_La of a system. Process scientists, engineers, and researchers may find value in considering directionality of the axial flow as a process parameter during their own upstream bioprocess characterization and development work. CLICK HERE TO VIEW THE WHITE PAPER.

Advancing the Robust Manufacture of T-Cell Therapies through the Application of Stirred Tank Bioreactors

Stirred-tank bioreactors present an option for the expansion of primary human T-Cells that has previously not been pursued due to concerns about shear stress. Small scale studies have shown that impeller driven mixing stirred-tank bioreactors do not increase cell death or limit expansion potential. Translating the learnings from those small scale studies to the autologous manufacturing scale has shown that the BIOne stirred-tank bioreactor produces a consistent culture environment and high cell yields. The BIOne significantly outperformed rocking bioreactor systems in both total yield and production efficiency. CLICK HERE TO VIEW THE POSTER.

Additional Resources

Additional resources are available in our Member Center. Click on the 'Sign In' button or Member Center on our navigation bar to be redirected. Registration is required to access the Member Center.

Request a Quote

Distek provides quotations on price and availability upon request. Quotations are processed by our Inside Sales Team 5 days a week from 8:30 to 5:00 EST. Typical turnaround for all quotes is 24-48 hours. Should you have any questions please direct them to orders@distekinc.com.

Contact Sales

Need Assistance?

Service and support from Distek is unparalleled in the industry. With over 40 years of experience, Distek is well-qualified to meet the unique challenges of your laboratory. Click the link below to request support.

Request Support